Below we have listed a collection of the latest publications of members of the Lipid Research Center.


Wiley CD, Davis S, Ramanathan A. Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry. Methods Mol Biol. 2019;1896:139-147


Ashe S, Malhotra V, Raghu P. Protein kinase D regulates metabolism and growth by controlling secretion of insulin like peptide. Dev Biol. 2018 Feb 1;434(1):175-185.

Balakrishnan SS, Basu U, Shinde D, Thakur R, Jaiswal M, Raghu P. Regulation of PI4P levels by PI4KIIIα during G-protein-coupled PLC signaling in Drosophila photoreceptors. J Cell Sci. 2018 Aug 3;131(15). pii: jcs217257.

Bonzón-Kulichenko E, Moltó E, Pintado C, Fernández A, Arribas C, Schwudke D, Gallardo N, Shevchenko A, Andrés A. Changes in Visceral Adipose Tissue Plasma Membrane Lipid Composition in Old Rats Are Associated With Adipocyte Hypertrophy With Aging. J Gerontol A Biol Sci Med Sci. 2018 Aug 10;73(9):1139-1146.

Brankatschk M, Gutmann T, Knittelfelder O, Palladini A, Prince E, Grzybek M, Brankatschk B, Shevchenko A, Coskun Ü, Eaton S. A temperature-dependent switch in feeding preference improves Drosophila development and survival in the cold. Dev Cell. 2018 Sep 24;46(6):781-793.e4.

Burla B, Arita M, Arita M, Bendt AK, Cazenave-Gassiot A, Dennis EA, Ekroos K, Han X, Ikeda K, Liebisch G, Lin MK, Loh TP, Meikle PJ, Orešič M, Quehenberger O, Shevchenko A, Torta F, Wakelam MJO, Wheelock CE, Wenk MR. MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines. J Lipid Res. 2018 Oct;59(10):2001-2017.

Chib S, Das S, Venkatesan S, Seshasayee ASN and Thattai M. Using stochastic cell division and death to probe minimal units of cellular replication. New J Phys. 2018, 20: 035004.

Cockcroft S, Raghu P. Phospholipid transport protein function at organelle contact sites. Curr Opin Cell Biol. 2018 Aug;53:52-60.

Ditlev JA, Vega AR, Köster DV, Su X, Tani T, Lakoduk AM, Vale RD, Mayor S, Jaqaman K, Rosen, MK. A Composition-Dependent Molecular Clutch Between T Cell Signaling Clusters and Actin. bioRxiv. 2018.

Freeman SA, Vega A, Riedl M, Collins RF, Ostrowski PP, Woods EC, Bertozzi CR, Tammi MI, Lidke DS, Johnson P, Mayor S, Jaqaman K, Grinstein S. Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement. Cell. 2018 Jan 11;172(1-2):305-317.e10.

Gao M, Maraspini R, Beutel O, Zehtabian A, Eickholt B, Honigmann A, Ewers H. Expansion Stimulated Emission Depletion Microscopy (ExSTED). ACS Nano. 2018 May 22;12(5):4178-4185.

Honigmann A, Nadler A. The Next Frontier: Quantitative Biochemistry in Living Cells. Biochemistry. 2018 Jan 9;57(1):47-55.

Hsu F, Spannl S, Ferguson C, Hyman AA, Parton RG, Zerial M. Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria. Elife. 2018 Feb 22;7.

Jaiman A and Thattai M. Algorithmic biosynthesis of eukaryotic glycans. bioRxiv, 2018,

Jha A, van Zanten TS, Philippe JM, Mayor S, Lecuit T. Quantitative Control of GPCR Organization and Signaling by Endocytosis in Epithelial Morphogenesis. Curr Biol. 2018 May 21;28(10):1570-1584.e6.

Knittelfelder O, Traikov S, Vvedenskaya O, Schuhmann A, Segeletz S, Shevchenko A, Shevchenko A. Shotgun Lipidomics Combined with Laser Capture Microdissection: A Tool To Analyze Histological Zones in Cryosections of Tissues. Anal Chem. 2018 Aug 21;90(16):9868-9878.

Kumar M, Joseph SR, Augsburg M, Bogdanova A, Drechsel D, Vastenhouw NL, Buchholz F, Gentzel M, Shevchenko A. MS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting. Mol Cell Proteomics. 2018 Feb;17(2):384-396.

Lehmann M, Knust E, Hebbar S. Drosophila melanogaster: A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa. Methods Mol Biol. 2019;1834:221-249.

Mitroulis I, Ruppova K, Wang B, Chen LS, Grzybek M, Grinenko T, Eugster A, Troullinaki M, Palladini A, Kourtzelis I, Chatzigeorgiou A, Schlitzer A, Beyer M, Joosten LAB, Isermann B, Lesche M, Petzold A, Simons K, Henry I, Dahl A, Schultze JL, Wielockx B, Zamboni N, Mirtschink P, Coskun Ü, Hajishengallis G, Netea MG, Chavakis T. Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity. Cell. 2018 Jan 11;172(1-2):147-161.e12

Panda A, Thakur R, Krishnan H, Naik A, Shinde D, Raghu P. Functional analysis of mammalian phospholipase D enzymes. Biosci Rep. 2018 Dec 7;38(6). pii:BSR20181690.

Parchure A, Vyas N, Mayor S. Wnt and Hedgehog: Secretion of Lipid-Modified Morphogens. Trends Cell Biol. 2018 Feb;28(2):157-170.

Rennert C, Vlaic S, Marbach-Breitrück E, Thiel C, Sales S, Shevchenko A, Gebhardt R, Matz-Soja M. The Diurnal Timing of Starvation Differently Impacts Murine Hepatic Gene Expression and Lipid Metabolism – A Systems Biology Analysis Using Self-Organizing Maps. Front Physiol. 2018 Sep 10;9:1180.

Sathe M, Muthukrishnan G, Rae J, Disanza A, Thattai M, Scita G, Parton RG, Mayor S. Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis. Nat Commun. 2018 May 9;9(1): 1835

Simons K. How Can Omic Science be Improved? Proteomics. 2018 Mar;18(5-6):e1800039

Suratekar R, Panda A, Raghu P, Krishna S. Evidence of sinks and sources in the phospholipase C-activated PIP(2) cycle. FEBS Lett. 2018 Mar;592(6):962-972.

Thottacherry JJ, Kosmalska AJ, Kumar A, Vishen AS, Elosegui-Artola A, Pradhan S, Sharma S, Singh PP, Guadamillas MC, Chaudhary N, Vishwakarma R, Trepat X, Del Pozo MA, Parton RG, Rao M, Pullarkat P, Roca-Cusachs P, Mayor S. Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells. Nat Commun. 2018 Oct 11;9(1):4217.

Thutupalli S, Geyer D, Singh R, Adhikari R, Stone HA. Flow-induced phase separation of active particles is controlled by boundary conditions. Proc Natl Acad Sci U S A. 2018 May 22;115(21):5403-5408.

Verboogen DRJ, Ter Beest M, Honigmann A, van den Bogaart G. Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules. FEBS Lett. 2018 May;592(9):1535-1544

Wagner N, Stephan M, Höglinger D, Nadler A. A Click Cage: Organelle-Specific Uncaging of Lipid Messengers. Angew Chem Int Ed Engl. 2018 Oct 1;57(40):13339-13343.

Yadav S, Thakur R, Georgiev P, Deivasigamani S, Krishnan H, Ratnaparkhi G, Raghu P. RDGBα localization and function at membrane contact sites is regulated by FFAT-VAP interactions. J Cell Sci. 2018 Jan 8;131(1). pii: jcs207985.


Anitei M, Stange C, Czupalla C, Niehage C, Schuhmann K, Sala P, Czogalla A, Pursche T, Coskun Ü, Shevchenko A, Hoflack B. Spatiotemporal Control of Lipid Conversion, Actin-Based Mechanical Forces, and Curvature Sensors during Clathrin/AP-1-Coated Vesicle Biogenesis. Cell Rep. 2017 Aug 29;20(9):2087-2099.

Bilgin M, Shevchenko A. Quantification of endogenous endocannabinoids by LC-MS/MS. In: Lipidomics. (Eds.) Paul Wood (Neuromethods ; 125).,New York,Springer (2017),99-107 Ch. 7.

Boland S, Schmidt U, Zagoriy V, Sampaio JL, Fritsche RF, Czerwonka R, Lübken T, Reimann J, Penkov S, Knölker HJ, Kurzchalia TV. Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans. Nat Chem Biol. 2017 Jun;13(6):647-654. doi: 10.1038/nchembio.2347. Epub 2017 Apr 3. PubMed PMID: 28369040.

Bowden JA, Heckert A, Ulmer CZ, Jones CM, Koelmel JP, Abdullah L, Ahonen L, Alnouti Y, Armando AM, Asara JM, Bamba T, Barr JR, Bergquist J, Borchers CH, Brandsma J, Breitkopf SB, Cajka T, Cazenave-Gassiot A, Checa A, Cinel MA, Colas RA, Cremers S, Dennis EA, Evans JE, Fauland A, Fiehn O, Gardner MS, Garrett TJ, Gotlinger KH, Han J, Huang Y, Neo AH, Hyötyläinen T, Izumi Y, Jiang H, Jiang H, Jiang J, Kachman M, Kiyonami R, Klavins K, Klose C, Köfeler HC, Kolmert J, Koal T, Koster G, Kuklenyik Z, Kurland IJ, Leadley M, Lin K, Maddipati KR, McDougall D, Meikle PJ, Mellett NA, Monnin C, Moseley MA, Nandakumar R, Oresic M, Patterson R, Peake D, Pierce JS, Post M, Postle AD, Pugh R, Qiu Y, Quehenberger O, Ramrup P, Rees J, Rembiesa B, Reynaud D, Roth MR, Sales S, Schuhmann K, Schwartzman ML, Serhan CN, Shevchenko A, Somerville SE, St John-Williams L, Surma MA, Takeda H, Thakare R, Thompson JW, Torta F, Triebl A, Trötzmüller M, Ubhayasekera SJK, Vuckovic D, Weir JM, Welti R, Wenk MR, Wheelock CE, Yao L, Yuan M, Zhao XH, Zhou S. Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma. J Lipid Res. 2017 Dec;58(12):2275-2288.

Ciucci S, Ge Y, Durán C, Palladini A, Jiménez-Jiménez V, Martínez-Sánchez LM, Wang Y, Sales S, Shevchenko A, Poser SW, Herbig M, Otto O, Androutsellis-Theotokis A, Guck J, Gerl MJ, Cannistraci CV. Enlightening discriminative network functional modules behind Principal Component Analysis separation in differential-omic science studies. Sci Rep. 2017 Mar 13;7:43946.

Frahnow T, Osterhoff MA, Hornemann S, Kruse M, Surma MA, Klose C, Simons K, Pfeiffer AFH. Heritability and responses to high fat diet of plasma lipidomics in a twin study. Sci Rep. 2017 Jun 16;7(1):3750.

Höglinger D, Nadler A, Haberkant P, Kirkpatrick J, Schifferer M, Stein F, Hauke S, Porter FD, Schultz C. Trifunctional lipid probes for comprehensive studies of single lipid species in living cells. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1566-1571.

Hwang S, Gustafsson HT, O’Sullivan C, Bisceglia G, Huang X, Klose C, Schevchenko A, Dickson RC, Cavaliere P, Dephoure N, Torres EM. Serine-Dependent Sphingolipid Synthesis Is a Metabolic Liability of Aneuploid Cells. Cell Rep. 2017 Dec 26;21(13):3807-3818

Kalappurakkal JM, Anilkumar AA, Patra C, van Zanten TS, Sheetz M, Mayor S. Acto-myosin driven functional nanoclusters of GPI-anchored proteins are generated by integrin receptor signaling. bioRxiv. 2017.

Kamalesh K, Trivedi D, Toscano S, Sharma S, Kolay S, Raghu P. Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics during clathrin-mediated endocytosis. J Cell Sci. 2017 Jul 1;130(13):2119-2133.

Koester D, Hundt N, Young GC, Fineberg A, Kukura P, Mayor S. Revealing intricate acto-myosin dynamics at a membrane surface using interferometric scattering microscopy. bioRxiv. 2017.

Liu G, Patch A, Bahar F, Yllanes D, Welch RD, Marchetti MC, Thutupalli S, Shaevitz JW. A motility-induced phase transition drives Myxococcus xanthus aggregation. 2017 arXiv:1709.06012 []

Maraspini R, Beutel O, Honigmann A. Circle scanning STED fluorescence correlation spectroscopy to quantify membrane dynamics and compartmentalization. Methods. 2018 May 1;140-141:188-197.

Metzendorf C, Zeigerer A, Seifert S, Sparla R, Najafi B, Canonne-Hergaux F, Zerial M, Muckenthaler MU. Acute loss of the hepatic endo-lysosomal system in vivo causes compensatory changes in iron homeostasis. Sci Rep. 2017 Jun 22;7(1):4023.

Meyer K, Ostrenko O, Bourantas G, Morales-Navarrete H, Porat-Shliom N, Segovia-Miranda F, Nonaka H, Ghaemi A, Verbavatz JM, Brusch L, Sbalzarini I, Kalaidzidis Y, Weigert R, Zerial M. A Predictive 3D Multi-Scale Model of Biliary Fluid Dynamics in the Liver Lobule. Cell Syst. 2017 Mar 22;4(3):277-290.e9.

Nordenfelt P, Moore TI, Mehta SB, Kalappurakkal JM, Swaminathan V, Koga N, Lambert TJ, Baker D, Waters JC, Oldenbourg R, Tani T, Mayor S, Waterman CM, Springer TA. Direction of actin flow dictates integrin LFA-1 orientation during leukocyte migration. Nat Commun. 2017 Dec 11;8(1):2047.

Ocaña-Morgner C, Sales S, Rothe M, Shevchenko A, Jessberger R. Tolerogenic versus Immunogenic Lipidomic Profiles of CD11c(+) Immune Cells and Control of Immunogenic Dendritic Cell Ceramide Dynamics. J Immunol. 2017 Jun 1;198(11):4360-4372.

Sadowski T, Klose C, Gerl MJ, Wójcik-Maciejewicz A, Herzog R, Simons K, Reich A, Surma MA. Large-scale human skin lipidomics by quantitative, high-throughput shotgun mass spectrometry. Sci Rep. 2017 Mar 7;7:43761.

Sales S, Knittelfelder O, Shevchenko A. Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry. Methods Mol Biol. 2017;1619:203-212.

Schuhmann K, Srzentić K, Nagornov KO, Thomas H, Gutmann T, Coskun Ü, Tsybin YO, Shevchenko A. Monitoring Membrane Lipidome Turnover by Metabolic (15)N Labeling and Shotgun Ultra-High-Resolution Orbitrap Fourier Transform Mass Spectrometry. Anal Chem. 2017 Dec 5;89(23):12857-12865.

Schuhmann K, Thomas H, Ackerman JM, Nagornov KO, Tsybin YO, Shevchenko A. Intensity-Independent Noise Filtering in FT MS and FT MS/MS Spectra for Shotgun Lipidomics. Anal Chem. 2017 Jul 5;89(13):7046-7052.

Schwudke D, Shevchenko A, Hoffmann N, Ahrends R. Lipidomics informatics for life-science. J Biotechnol. 2017 Nov 10;261:131-136.

Sellin J, Schulze H, Paradis M, Gosejacob D, Papan C, Shevchenko A, Psathaki OE, Paululat A, Thielisch M, Sandhoff K, Hoch M. Characterization of Drosophila Saposin-related mutants as a model for lysosomal sphingolipid storage diseases. Dis Model Mech. 2017 Jun 1;10(6):737-750.

Sezgin E, Azbazdar Y, Ng XW, Teh C, Simons K, Weidinger G, Wohland T, Eggeling C, Ozhan G. Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments. FEBS J. 2017 Aug;284(15):2513-2526.

Sezgin E, Levental I, Mayor S, Eggeling C. The mystery of membrane organization: composition, regulation and roles of lipid rafts. Nat Rev Mol Cell Biol. 2017 Jun;18(6):361-374.

Shevchenko A, Yang Y, Knaust A, Verbavatz JM, Mai H, Wang B, Wang C, Shevchenko A. Open sesame: Identification of sesame oil and oil soot ink in organic deposits of Tang Dynasty lamps from Astana necropolis in China. PLoS One. 2017 Feb 24;12(2):e0158636.

Simons K. Coming to grips with cell surface polarity. Nat Rev Mol Cell Biol. 2017 May;18(5):278.

Shukla A, Bhattacharyya A, Kuppusamy L, Srivas M, Thattai M. Discovering vesicle traffic network constraints by model checking. PLoS One. 2017 Jul 6;12(7):e0180692

Spannl S, Kumichel A, Hebbar S, Kapp K, Gonzalez-Gaitan M, Winkler S, Blawid R, Jessberger G, Knust E. The Crumbs_C isoform of Drosophila shows tissue- and stage-specific expression and prevents light-dependent retinal degeneration. Biol Open. 2017 Feb 15;6(2):165-175.

Stefanko A, Thiede C, Ehninger G, Simons K, Grzybek M. Lipidomic approach for stratification of acute myeloid leukemia patients. PLoS One. 2017 Feb 16;12(2):e0168781.

Steringer JP, Lange S, Čujová S, Šachl R, Poojari C, Lolicato F, Beutel O, Müller HM, Unger S, Coskun Ü, Honigmann A, Vattulainen I, Hof M, Freund C, Nickel W. Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components. Elife. 2017 Jul 19;6. pii: e28985.

Swaminathan V, Kalappurakkal JM, Mehta SB, Nordenfelt P, Moore TI, Koga N, Baker DA, Oldenbourg R, Tani T, Mayor S, Springer TA, Waterman CM. Actin retrograde flow actively aligns and orients ligand-engaged integrins in focal adhesions. Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):10648-10653.

Taylor MJ, Husain K, Gartner ZJ, Mayor S, Vale RD. A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering in Ligand Discrimination. Cell. 2017 Mar 23;169(1):108-119.e20.

Thattai M. Organelle acidification: an ancient cellular leak detector. BMC Biol. 2017 Jun 26;15(1):51

Thutupalli S, Uppaluri S, Constable GW, Levin SA, Stone HA, Tarnita CE, Brangwynne CP. Farming and public goods production in Caenorhabditis elegans populations. Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2289-2294.

Walter AM, Müller R, Tawfik B, Wierda KD, Pinheiro PS, Nadler A, McCarthy AW, Ziomkiewicz I, Kruse M, Reither G, Rettig J, Lehmann M, Haucke V, Hille B, Schultz C, Sørensen JB. Phosphatidylinositol 4,5-bisphosphate optical uncaging potentiates exocytosis. Elife. 2017 Oct 25;6.

Zee T, Bose N, Zee J, Beck JN, Yang S, Parihar J, Yang M, Damodar S, Hall D, O’Leary MN, Ramanathan A, Gerona RR, Killilea DW, Chi T, Tischfield J, Sahota A, Kahn A, Stoller ML, Kapahi P. α-Lipoic acid treatment prevents cystineurolithiasis in a mouse model of cystinuria. Nat Med. 2017 Mar;23(3):288-290.

Zeigerer A, Wuttke A, Marsico G, Seifert S, Kalaidzidis Y, Zerial M. Functional properties of hepatocytes in vitro are correlated with cell polarity maintenance. Exp Cell Res. 2017 Jan 1;350(1):242-252.

Zuidscherwoude M, Dunlock VE, van den Bogaart G, van Deventer SJ, van der Schaaf A, van Oostrum J, Goedhart J, In ‘t Hout J, Hämmerling GJ, Tanaka S, Nadler A, Schultz C, Wright MD, Adjobo-Hermans MJW, van Spriel AB. Tetraspanin microdomains control localized protein kinase C signaling in B cells. Sci Signal. 2017 May 9;10(478).