Arvind Ramanathan

INSTEM | Group Leader

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Project Description

The technologies for single cell metabolomics are coming of age using novel cell sampling and sensitive mass spectrometry approaches. The two more widely using technological approaches to date are Microarray for Mass Spectrometry (MAMS) and Capillary Electrophoresis- Mass Spectrometry.
Dr. Ramanathan’s group is interested in studying single cell metabolomics of skeletal muscle satellite calls and a developing embryo. We will isolate pax 7+ve satellite cells from mice. We will also culture developing zebrafish embryos and collect them at various cell stages up to the 64-cell stage and analyze both lipid and aqueous metabolites.
We plan to develop the ability to perform metabolomics on live systems without fixation, solvent extraction and drying. Therefore, open questions in satellite cell activation, and  embryonic development including the yet unknown underlying mechanisms of embryonic mitochondrial segregation and quality control will become possible to answer using this technology.

  • Collection of Lipid Center relevant Publications

    Wiley CD, Davis S, Ramanathan A. Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry. Methods Mol Biol. 2019;1896:139-147

    Zee T, Bose N, Zee J, Beck JN, Yang S, Parihar J, Yang M, Damodar S, Hall D, O’Leary MN, Ramanathan A, Gerona RR, Killilea DW, Chi T, Tischfield J, Sahota A, Kahn A, Stoller ML, Kapahi P. α-Lipoic acid treatment prevents cystineurolithiasis in a mouse model of cystinuria. Nat Med. 2017 Mar;23(3):288-290.

    Mark KA, Dumas KJ, Bhaumik D, Schilling B, Davis S, Oron TR, Sorensen DJ, Lucanic M, Brem RB, Melov S, Ramanathan A, Gibson BW, Lithgow GJ. Vitamin D Promotes Protein Homeostasis and Longevity via the Stress Response Pathway Genes skn-1, ire-1, and xbp-1. Cell Rep. 2016 Oct 25;17(5):1227-1237

    Chaudhuri J, Bose N, Gong J, Hall D, Rifkind A, Bhaumik D, Peiris TH, Chamoli M, Le CH, Liu J, Lithgow GJ, Ramanathan A, Xu XZS, Kapahi P. A Caenorhabditis elegans Model Elucidates a Conserved Role for TRPA1-Nrf Signaling in Reactive α-Dicarbonyl Detoxification. Curr Biol. 2016 Nov 21;26(22):3014-3025

    S Sonnet D, N O’Leary M, A Gutierrez M, M Nguyen S, Mateen S, Hsu Y, P Mitchell K, J Lopez A, Vockley J, K Kennedy B, Ramanathan A. Metformin inhibits Branched Chain Amino Acid (BCAA) derived ketoacidosis and promotes metabolic homeostasis in MSUD. Sci Rep. 2016 Jul 4;6:28775

    Wiley CD, Velarde MC, Lecot P, Liu S, Sarnoski EA, Freund A, Shirakawa K, Lim HW, Davis SS, Ramanathan A, Gerencser AA, Verdin E, Campisi J. Mitochondrial Dysfunction Induces Senescence with a Distinct Secretory Phenotype. Cell Metab. 2016 Feb 9;23(2):303-14

    Katewa SD, Akagi K, Bose N, Rakshit K, Camarella T, Zheng X, Hall D, Davis S, Nelson CS, Brem RB, Ramanathan A, Sehgal A, Giebultowicz JM, Kapahi P. Peripheral Circadian Clocks Mediate Dietary Restriction-Dependent Changes in Lifespan and Fat Metabolism in Drosophila. Cell Metab. 2016 Jan 12;23(1):143-54

    Chi T, Kim MS, Lang S, Bose N, Kahn A, Flechner L, Blaschko SD, Zee T, Muteliefu G, Bond N, Kolipinski M, Fakra SC, Mandel N, Miller J, Ramanathan A, Killilea DW, Brückner K, Kapahi P, Stoller ML. A Drosophila model identifies a critical role for zinc in mineralization for kidney stone disease. PLoS One. 2015 May 13;10(5):e0124150