Structure of membrane
Membranes play pivotal role in cellular functions in creating barriers and acting as molecular sieves. Many of the cellular functions involves the expansion, fission and fusion of membranes. We are interested in studying the process of vesicle fusion in the formation of early endosomes. Early endosome antigen 1 (EEA1) is believed to be an important protein which brings the vesicles closer. Together with the groups of Prof. Marino Zerial and Prof. Madan Rao, we are studying the dynamics of EEA1 on the membranes. To probe the dynamics of EEA1 on the membranes we use fluorescence correlation spectroscopy (FCS) and total internal reflection fluorescence microscopy to study the binding kinetics on membranes, which are created using microfluidics technology. Further, we use the same experimental setting to study the effects of crowding by the presence of other molecules on membranes.
Thutupalli S, Geyer D, Singh R, Adhikari R, Stone HA. Flow-induced phase separation of active particles is controlled by boundary conditions. Proc Natl Acad Sci U S A. 2018 May 22;115(21):5403-5408
Thutupalli S, Uppaluri S, Constable GW, Levin SA, Stone HA, Tarnita CE, Brangwynne CP. Farming and public goods production in Caenorhabditis elegans populations. Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2289-2294.
Olmi S, Martens EA, Thutupalli S, Torcini A. Intermittent chaotic chimeras for coupled rotators. Phys Rev E Stat Nonlin Soft Matter Phys. 2015 Sep;92(3):030901
Thutupalli S, Sun M, Bunyak F, Palaniappan K, Shaevitz JW. Directional reversals enable Myxococcus xanthus cells to produce collective one-dimensional streams during fruiting-body formation. J R Soc Interface. 2015 Aug 6;12(109):20150049