Sunil Laxman

INSTEM | Group Leader

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  • Basic Research


Project Description

The Laxman lab has an active, ongoing collaboration with the laboratory of Prof. Teymuras Kurzchalia at the MPI-CBG. We are together addressing molecular mechanisms of cryptobiosis/anhydrobiosis, or life without water. In particular, we are addressing how specific metabolites, and switching to certain metabolic states determines the ability of cells to survive extreme loss of water. To address this, we use two distinct model organisms (S. cerevisiae and C. elegans), and a range of complimentary experimental approaches. The Kurzchalia lab brings substantial expertise in lipid metabolism and nematode genetics, biochemistry and cell biology. The Laxman lab has expertise in amino acid and carbohydrate metabolism, metabolic flux analysis and S. cerevisiae biochemistry, genetics and cell biology. Our first joint effort was the discovery of how an ignored biochemical pathway, the glyoxylate shunt, was critical for the production of a trehalose, and for desiccation tolerance (Erkut et al, eLIFE 2016). Through a jointly supported postdoctoral fellow (Akshay Das Adhikari), we are currently exploring other metabolic nodes that are critical for desiccation tolerance, as well as identifying their cellular and molecular mechanisms of action. The next sets of stories are nearing completion. Collectively, we are addressing how specific metabolites (coming from lipid and amino acid metabolism) can determine if cells can survive extreme water loss.

  • Collection of Lipid Center relevant Publications

    Walvekar AS, Srinivasan R, Gupta R, Laxman S. Methionine coordinates a hierarchically organized anabolic program enabling proliferation. Mol Biol Cell. 2018 Oct 24:mbcE18080515. doi: 10.1091/mbc.E18-08-0515. [Epub ahead of print]

    Walvekar A, Rashida Z, Maddali H, Laxman S. A versatile LC-MS/MS approach for comprehensive, quantitative analysis of central metabolic pathways. Wellcome Open Res. 2018 Sep 20;3:122. doi: 10.12688/wellcomeopenres.14832.1. eCollection 2018.

    Krishna S, Laxman S. A minimal “push-pull” bistability model explains oscillations between quiescent and proliferative cell states. Mol Biol Cell. 2018 Sep 15;29(19):2243-2258

    Laxman S. Conceptualizing Eukaryotic Metabolic Sensing and Signaling. J Indian Inst Sci. 2017 Mar;97(1):59-77.

    Deshpande AA, Bhatia M, Laxman S, Bachhawat AK. Thiol trapping and metabolic redistribution of sulfur metabolites enable cells to overcome cysteine overload. Microb Cell. 2017 Mar 27;4(4):112-126

    Erkut C, Gade VR, Laxman S, Kurzchalia TV. The glyoxylate shunt is essential for desiccation tolerance in C. elegans and budding yeast. Elife. 2016 Apr 19;5. pii: e13614

    Sato S, Jung H, Nakagawa T, Pawlosky R, Takeshima T, Lee WR, Sakiyama H, Laxman S, Wynn RM, Tu BP, MacMillan JB, De Brabander JK, Veech RL, Uyeda K. Metabolite Regulation of Nuclear Localization of Carbohydrate-response Element-binding Protein (ChREBP): ROLE OF AMP AS AN ALLOSTERIC INHIBITOR. J Biol Chem. 2016 May 13;291(20):10515-27

    Dhawan J, Laxman S. Decoding the stem cell quiescence cycle–lessons from yeast for regenerative biology. J Cell Sci. 2015 Dec 15;128(24):4467-74

    Dutchak PA, Laxman S, Estill SJ, Wang C, Wang Y, Wang Y, Bulut GB, Gao J, Huang LJ, Tu BP. Regulation of Hematopoiesis and Methionine Homeostasis by mTORC1 Inhibitor NPRL2. Cell Rep. 2015 Jul 21;12(3):371-9